Plant Transcription Factor Database
Previous version: v3.0
Petunia inflata
HSF Family
Species TF ID Description
Peinf101Scf00035g04012.1HSF family protein
Peinf101Scf00066g02042.1HSF family protein
Peinf101Scf00146g18036.1HSF family protein
Peinf101Scf00167g10009.1HSF family protein
Peinf101Scf00199g19020.1HSF family protein
Peinf101Scf00227g02016.1HSF family protein
Peinf101Scf00372g03018.1HSF family protein
Peinf101Scf00434g11030.1HSF family protein
Peinf101Scf00447g03026.1HSF family protein
Peinf101Scf00482g08063.1HSF family protein
Peinf101Scf00569g10009.1HSF family protein
Peinf101Scf00571g08015.1HSF family protein
Peinf101Scf00713g03004.1HSF family protein
Peinf101Scf00961g04004.1HSF family protein
Peinf101Scf00962g03023.1HSF family protein
Peinf101Scf01033g00010.1HSF family protein
Peinf101Scf01046g13010.1HSF family protein
Peinf101Scf01048g02004.1HSF family protein
Peinf101Scf01099g01019.1HSF family protein
Peinf101Scf01136g07007.1HSF family protein
Peinf101Scf01151g00014.1HSF family protein
Peinf101Scf01157g00001.1HSF family protein
Peinf101Scf01199g00013.1HSF family protein
Peinf101Scf01452g13033.1HSF family protein
Peinf101Scf01482g04034.1HSF family protein
Peinf101Scf01544g01011.1HSF family protein
Peinf101Scf01853g03001.1HSF family protein
Peinf101Scf02093g03017.1HSF family protein
Peinf101Scf02707g01029.1HSF family protein
Peinf101Scf03556g00033.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645